This book constitutes the refereed proceedings of the 14th Asian Symposium on Programming Languages and Systems, APLAS 2016, held in Hanoi, Vietnam, in November 2016. The papers cover a variety of topics such as semantics, logics, and foundational theory; design of languages type systems, and foundational calculi; domain-specific languages; compilers, interpreters, and abstract machines; program derivation, synthesis and transformation; program analysis, verification, and model-checking; logic, constraint, probabilistic and quantum programming; software security; concurrency and parallelism; tools for programming and implementation.