The 2016 International Conference on Artificial Intelligence Science and Technology (AIST2016) was held in Shanghai, China, from 15th to 17th July, 2016.AIST2016 aims to bring together researchers, engineers, and students to the areas of Artificial Intelligence Science and Technology. AIST2016 features unique mixed topics of artificial intelligence and application, computer and software, communication and network, information and security, data mining, and optimization.This volume consists of 101 peer-reviewed articles by local and foreign eminent scholars which cover the frontiers and state-of-art development in AI Technology.