This book outlines the practical applications of microbial nanomaterials in healthcare with an easy-to-understand approach. The first chapter of the book summarizes the biosynthesis of microbial nanomaterials and the various strategies involved in the process. Subsequent chapters delve into the real-world applications of these nanomaterials, including their role in combating antimicrobial resistance, treating multi-drug-resistant bacterial infections and enhancing the precision of targeting antibiotic-resistant microbes through artificial intelligence. Additionally, the book includes studies related to the potential of microbial nanomaterials for novel cancer therapies, revolutionizing diagnostics and imaging, sustainable agriculture, plant disease control, toxicant mitigation, nanobiosensing and water purification. Finally, the last chapter familiarizes the reader with the difficulties and challenges associated with the production of the microbial nanomaterials. The book is a valuable reference for researchers, the scientific community, academicians and the medical practitioners and helps readers become aware of the utilities of microbial nanomaterials in the biomedical arena.