DNA delivery into cells is a rapidly developing area in gene therapy and biotechnology. The use of histidine-rich peptides and polypeptides as DNA delivery systems and self-assembled delivery systems based on cationic lipids and polymers are discussed.

I: Introduction; D. Luo, W.M. Saltzman. II: Overcoming Cellular Barriers along the DNA Delivery Pathway. Outside of the cell: Supramolar assemblies of DNA delivery systems; Pitard. At the cell surface: Engineering the surface properties of synthetic gene delivery systems; Schaffer. Inside of the cell: Histidine containing peptides and polypeptides as nucleic acid vectors; Midoux. Intracellular targets for DNA delivery: nuclei and mitochondria; Torchilin. III: Targeted DNA Delivery. Immune cells: Mannose receptor-mediated gene delivery into antigen presenting dendritic cells; Zenke. Muscle: Electro-gene-transfer: a new approach for muscle gene delivery; Toniatti. Tumor: Tumor-targeted gene transfer with DNA polyplexes; Wagner. IV: Novel DNA Delivery Systems. Light: Photochemical transfection: a technology for efficient light directed gene delivery; Hogset. Ultrasound: Sonoportation: mechanical DNA delivery by ultrasonic cavitation; Miller, Greenleaf.

GPSR Compliance The European Union's (EU) General Product Safety Regulation (GPSR) is a set of rules that requires consumer products to be safe and our obligations to ensure this. If you have any concerns about our products you can contact us on ProductSafety@springernature.com. In case Publisher is established outside the EU, the EU authorized representative is: Springer Nature Customer Service Center GmbH Europaplatz 3 69115 Heidelberg, Germany ProductSafety@springernature.com