Preface xv 1 Discovery of Novel and Biologically Active Compounds from Algae 1 M. Singh, N. Gupta, P. Gupta, Doli, P. Mishra and A. Yadav 1.1 Introduction 2 1.2 Microalgae-Derived Natural Products 3 1.3 Bioprospecting for New Algae 4 1.4 Therapeutically Essential Natural Products 6 1.5 Screening for Bioactive Constituents 7 1.6 Extraction Methods 9 1.7 Biosynthesis and Biological Activities 11 1.7.1 Antibacterial Action 15 1.7.2 Antifungal Action 17 1.7.3 Anti-Inflammatory Action 18 1.7.4 Antiprotozoal Action 19 1.7.5 Antioxidant Action 20 1.7.6 Antineoplastic (Anticancer) Action 21 1.7.7 Antiviral Action 24 1.7.8 Anticoagulant Action 25 1.7.9 Immunosuppressive Action 25 1.8 Conclusion 26 References 27 2 Bioactive Compounds Synthesized by Algae: Current Development and Prospects as Biomedical Application in the Pharmaceutical Industry 41 Preeti Mishra, Namrata Gupta, Monika Singh and Deeksha Tiwari 2.1 Introduction 42 2.2 Algal-Sourced Compounds of Medical Interest 43 2.3 Microalgae with Potential for Obtaining Bioactive Compounds 44 2.3.1 Spirulina 46 2.3.2 Chlorella 47 2.3.3 Nostoc 49 2.3.4 Dunaliella 50 2.4 Bioactive Compounds from Cyanobacteria 51 2.5 Secondary Metabolites from Microalgae 55 2.5.1 Carotenoids 55 2.5.1.1 β-Carotene 55 2.5.1.2 Astaxanthin 57 2.5.1.3 Zeaxanthin and Lutein 58 2.5.1.4 Violaxanthin 59 2.5.1.5 Fucoxanthin 59 2.5.2 Polyunsaturated Fatty Acids 60 2.5.3 Proteins and Polypeptides 61 2.6 Biomass of Microalgae 62 2.6.1 Biomass Production 62 2.6.1.1 Cultivation 62 2.6.1.2 Harvesting 63 2.6.1.3 Biomass Dehydration 64 2.6.1.4 Extraction of Bioactive Compounds 66 2.7 Pharmaceutical Applications of Microalgae 66 2.8 Conclusion 71 References 72 3 Bioactive Compounds Derived from Microalgae Showing Diverse Medicinal Activities 77 D. Tiwari, P. Mishra and N. Gupta 3.1 Introduction 78 3.2 Microalgae with Anti-Inflammatory Activity 81 3.3 Microalgae with Immunomodulatory Activity 82 3.4 Microalgae Anticancer Activity 85 3.5 Potential of Microalgae in Quality Enhancement of Natural Products 87 3.5.1 Pharmaceutical Industry 87 3.5.2 Cosmetics and Personal Care 87 3.5.3 Food Industry 88 References 90 4 Application of Astaxanthin and Carotenoids Derived from Algae for the Production of Nutraceuticals, Pharmaceuticals, Additives, Food Supplement and Feed 95 Abiola Folakemi Olaniran, Joshua Opeyemi Folorunsho, Bolanle Adenike Akinsanola, Abiola Ezekiel Taiwo, Yetunde Mary Iranloye, Clinton Emeka Okonkwo and Omorefosa Osarenkhoe Osemwegie 4.1 Carotenoids and Its Characteristics 96 4.1.1 Sources of Carotenoids 97 4.1.2 Production/Extraction of Carotenoids 99 4.2 Astaxanthin and Its Characteristics 102 4.2.1 Production/Extraction of Astaxanthin 103 4.2.2 Historical Perspective of Consumption of Alga as Food and Utilization in the Food Industry 104 4.3 Application/Utilization of Astaxanthin and Carotenoids in Different Sectors 105 4.3.1 Nutraceuticals 108 4.3.2 Food Additives, Supplements and Feed Formulation 110 4.3.3 Alga as a Potential Source of Astaxanthin and Food Supplement 111 4.3.4 Technological Application of Algae as Origins of Supplements and Bioactive Mixtures in Healthier Food Varieties and Drinks 112 4.3.5 Enriching Dairy Products with Algae 114 4.3.6 Algae as a Potential Healthy Protein and Fat Source 115 4.4 Future Perspective 117 References 118 5 Production of Polyunsaturated Fatty Acids (PUFAs) and Their Biomedical Application 125 Olorunsola Adeyomoye, Olugbemi T. Olaniyan and Charles O. Adetunji 5.1 Introduction 125 5.2 Polyunsaturated Fatty Acids 126 5.3 Production of Polyunsaturated Fatty Acids 127 5.4 Nanomedicine-Based Formulations Containing Polyunsaturated Fatty Acids 128 5.5 Biological and Medical Application of Polyunsaturated Fatty Acids 129 5.6 Metabolism of Polyunsaturated Fatty Acid 131 5.7 Challenges and Issues of Production and Use of Polyunsaturated Fatty Acids 132 5.8 Conclusion 133 References 133 6 Utilization of Algae and Their Anti-Proliferative and Anti-Inflammatory Activities 139 Olorunsola Adeyomoye, Olugbemi T. Olaniyan and Charles O. Adetunji 6.1 Introduction 140 6.2 Physiology and Biochemistry of Algae 141 6.3 Algae Biocomposites 141 6.4 Techniques and Methods Involved in the Production of Algae Biocomposites 143 6.5 Antiproliferative Activities of Algae 144 6.6 Anti-Inflammatory Activities of Algae 144 6.7 Potential Health Benefits of Algae Biocomposites 146 6.8 Challenges and Issues Related to Algae Biocomposites Use 147 6.9 Conclusion 147 References 148 7 Natural Compounds of Algae Origin with Potential Anticarcinogenic Benefits 153 Adewale Omowumi Oyeronke, Asowata-Ayodele Abiola Mojisola, Akomolafe Seun Funmilola and Adetunji Juliana Bunmi 7.1 Introduction 154 7.2 Progression, Predisposing Factors and Treatment of Cancer 156 7.2.1 Cancer Progression 156 7.2.2 Predisposing Factors to Cancer 157 7.2.3 Treatment of Cancer 157 7.3 Features of Microalgae 157 7.4 Sources of Microalgae 158 7.5 Fractions of Microalgae Species with Anticancer Properties 158 7.5.1 Carotenoid-Rich Extracts of Chlorella Species 158 7.5.2 Chaetoceros Calcitrans Ethyl Acetate and Ethanol Extracts 159 7.5.3 Amphidinium Carterae Organic Fractions 159 7.5.4 Methanolic Extracts from Amphidinium Carterae, Prorocentrum Rhathymum, Symbiodinium sp., Coolia Malayensis, Ostreopsis Ovata, Amphidinium Operculatum, and Heterocapsa Psammophila 160 7.5.5 Skeletonema Marinoi Hydrophobic Fraction 160 7.5.6 Canadian Marine Microalgal Pool Aqueous Extract 160 7.5.7 Chlorella Sorokiniana Aqueous Extract 161 7.6 Compounds with Anticarcinogenic Activities Isolated from Marine Microalgae 161 7.6.1 Polysaccharides 161 7.6.2 Phycocyanin 163 7.6.3 Chlorophyll 163 7.6.4 Polyunsaturated Aldehydes (PUAs) 164 7.6.5 Violaxanthin 164 7.6.6 Eicosapentaenoic Acid (EPA) 165 7.6.7 Stigmasterol 166 7.6.8 Fucoxanthin 166 7.6.9 Nonyl 8-Acetoxy-6-Methyloctanoate (NAMO) 167 7.6.10 Monogalactosyl Glycerols 168 7.6.11 Other Active Compounds from Microalgae with Anticarcinogenic Activities 168 7.7 Conclusion and Recommendation 168 References 169 8 Current Research on Algal-Derived Sulfated Polysaccharides and Their Antiulcer Bioactivities 177 Abiola Mojisola Asowata-Ayodele, Adewale Omowumi Oyeronke, Akomolafe Seun Funmilola and Adetunji Juliana Bunmi 8.1 Introduction 178 8.1.1 Symptoms of Peptic Ulcer Disease 179 8.2 Treatment Using Synthetic Medicines 181 8.3 Natural Products Used in the Treatment of Peptic Ulcer 183 8.4 Antiulcer Products Developed from Algae 184 8.4.1 Phycocolloids 186 8.4.2 Fucoidan 188 8.4.3 Ulvans 189 8.4.4 Laminaran 190 8.4.5 Xylan and Porphyran 191 8.5 Conclusion 193 References 193 9 Pharmacological and Antioxidant Attributes of Significant Bioactives Constituents Derived from Algae 197 Juliana Bunmi Adetunji, Abigail Omotayo Agbolade, Omowumi Oyeronke Adewale, Ikechukwu P. Ejidike, Charles Oluwaseun Adetunji and Isreal Olu Oyewole 9.1 Introduction 198 9.1.1 Brown Algae 198 9.1.1.1 Fucoidan and Its Bioactivity 198 9.1.1.2 Benefits Derived from Fucoidan 200 9.1.1.3 Laminarin 202 9.1.1.4 Fucosterol 204 9.1.1.5 Saccharides 204 9.1.1.6 Phlorotannins 206 9.1.1.7 Dieckol 207 9.1.2 Red Algae 207 9.1.2.1 D-Isofloridoside 207 9.1.2.2 Phycoerythrin 208 9.1.3 Blue-Green Algae 209 9.1.3.1 Phycocyanin and Phycocyanobilin 209 9.1.4 Other Potential Applications of Algae 215 9.1.4.1 Antioxidant and Anti-Tyrosine Capabilities 215 9.2 Conclusion 216 References 216 10 Utilization of Pharmacologically Relevant Compounds Derived from Algae for Effective Management of Diverse Diseases 223 Olulope Olufemi Ajayi 10.1 Introduction 223 10.2 Algae in the Management of Some Diseases 225 10.2.1 Cancer 225 10.2.2 Inflammatory Bowel Disease 226 10.2.3 Osteoarthritis 227 10.2.4 Gastric Ulcers 227 10.2.5 Neurodegenerative Diseases 227 10.2.6 Diabetes Mellitus 228 10.2.7 Hypertension 228 10.2.8 Atherosclerosis 229 10.2.9 Kidney and Liver Diseases 230 10.2.10 Skin Diseases/Disorders 230 10.2.11 Uterine Leiomyomas 231 10.2.12 Obesity 232 10.2.13 Tuberculosis 234 10.2.14 Asthma 235 10.2.15 Hepatitis 236 10.3 Xanthophylls 236 10.3.1 Astaxanthin 236 10.3.2 Fucoxanthin 237 10.3.3 Lutein and Zeaxanthin 237 10.3.4 Beta-Cryptoxanthin 237 10.3.5 Siphonaxanthin 238 10.3.6 Saproxanthin and Myxol 238 10.4 Alga Diterpenes 238 10.5 Conclusion 239 References 239 11 Application of Algae in Wound Healing 251 Ebenezer I. O. Ajayi, Johnson O. Oladele and Abraham O. Nkumah 11.1 Introduction 252 11.1.1 Current Trends in the Design of Wound Dressings 253 11.2 Brown Seaweed Polysaccharides 256 11.2.1 Fucoidan 257 11.2.2 Alginate 258 11.2.3 Carrageenan 259 11.2.4 Red Seaweed Polysaccharides 260 11.2.5 Green Seaweed Polysaccharides 260 11.3 Mechanisms Underpinning the Wound Healing Effects of Algae 261 11.3.1 Hemostatic Activity 263 11.3.2 Immunomodulatory and Anti-Inflammatory Effects 264 11.3.3 Antioxidant Activity 267 11.3.4 Antifungal Activity 269 11.3.5 Antibacterial Properties 269 11.3.6 Wound-Healing Property of Algae and Cyanobacteria 271 11.4 Conclusion 274 References 274 12 Application of Nanotechnology for the Bioengineering of Useful Metabolites Derived from Algae and Their Multifaceted Applications 285 Charles Oluwaseun Adetunji, Olugbemi T. Olaniyan, Inobeme Abel, Ruth Ebunoluwa Bodunrinde, Nyejirime Young Wike, Wadzani Dauda Palnam, Juliana Bunmi Adetunji, Phebean Ononsen Ozolua, Arshad Farid, Shakira Ghazanfar, Olorunsola Adeyomoye, Muhammad Akram, Chibuzor Victory Chukwu and Mohammed Bello Yerima 12.1 Introduction 286 12.2 Various Types of Nanoparticles Derived from Algae 287 12.3 Nanoparticles from Algae and the Key Role They Play in the Medical and Pharmaceutical Sectors 295 12.3.1 Anticancer Activity 298 12.4 Algae-Derived Nanoparticles and Their Key Role in the Cosmetics Industry 302 12.4.1 Algae-Derived Nanoparticles as Moisturizer 302 12.4.2 Algae-Derived Nanoparticles as Skin Sensitizing and Thickening Agents 302 12.4.3 Algae-Derived Nanoparticles as Anti-Aging Agents 303 12.4.4 Algae-Derived Nanoparticles as Antioxidant Agent 303 12.5 Algae-Derived Nanoparticles as Antibacterial Agent 303 12.6 Algae-Derived Nanoparticles as Antifungal Agent 306 12.7 Algae-Derived Nanoparticles as Antiviral Agent 306 12.8 Conclusion 307 References 307 13 Discovery of Novel Compounds of Pharmaceutical Significance Derived from Algae 321 Charles Oluwaseun Adetunji, Muhammad Akram, Fahad Said Khan, Olugbemi T. Olaniyan, Babatunde Oluwafemi Adetuyi, Inobeme Abel, Ruth Ebunoluwa Bodunrinde, Juliana Bunmi Adetunji, Phebean Ononsen Ozolua, Nyejirime Young Wike, Wadzani Dauda Palnam, Arshad Farid, Shakira Ghazanfar, Olorunsola Adeyomoye, Chibuzor Victory Chukwu and Mohammed Bello Yerima 13.1 Introduction 322 13.2 Bioactive Compounds 323 13.3 Pharmacological Significance of Algae 324 13.3.1 Antioxidative Activity 324 13.3.2 Antihypertensive Activity 325 13.3.3 Anticoagulant Activity 326 13.3.4 Antiproliferation Activities 326 13.3.5 Immune-Stimulant Activity 327 13.3.6 Cholesterol-Lowering Activity 328 13.3.7 Anti-Inflammatory Activity 329 13.3.8 Anticancer Activity 329 13.3.9 Cancer Prevention Agent 330 13.3.10 Antidiabetic 330 13.3.11 Different Biomedical Activities 331 13.4 Research Results on Well-Studied Algal Strains 332 13.5 Conclusion and Future Recommendations 334 References 334 14 Applications of Algae in the Production of Single-Cell Proteins and Pigments with High Relevance in Industry 343 Juliana Bunmi Adetunji, Omowumi Oyeronke Adewale, Charles Oluwaseun Adetunji and Isreal Olu Oyewole 14.1 Introduction 344 14.2 Microalgae-Derived Single Cell Protein (SCP) 345 14.2.1 Dunaliella 346 14.3 Applications of SCP in Diets 347 14.4 Pigments Derived from Algae 348 14.4.1 Astaxanthin 348 14.4.2 Fucoxanthin 348 14.4.3 Carotenoids 349 14.5 Conclusion 349 References 349 Index 353

The book comprehensively details the novel and biologically active compounds derived from algae for sustainable healthcare delivery that could be used for the treatment of an ever-increasing population, prevention of high rate of morbidity rates, as well as in the treatment of numerous diseases, and serve as an alternative drug for the prevention of high level of resistance to synthetic drugs. This second volume places a special emphasis on the discovery of novel and biologically active compounds from algae. It covers a wide range of applications, including the use of astaxanthin and carotenoids derived from algae for the production of nutraceuticals, pharmaceuticals, additives, food supplements, and feed. The book also discusses the production of polyunsaturated fatty acids (PUFAs) and their biomedical applications, recent advancements in the research of sulfated polysaccharides from algal origin, and their antiulcer bioactivities. Other topics include the application of algae in wound healing, the use of nanotechnology for the bioengineering of useful metabolites derived from algae and their multifaceted applications, and the production of single-cell proteins and pigments with high relevance in the industry. Audience Researchers in industry and academia as well as clinicians in the fields of microbiology, biotechnology, and food science will find this book very pertinent.