1. An introduction to advanced technologies in synthetic biology Vijai Singh, Gargi Bhattacharjee, Nisarg Gohil, Rupesh Maurya, Navya L. Lam and Khalid J. Alzahrani 2. Design and characterization of synthetic promoters Boris Kirov and Slavil Peykov 3. Recent development in DNA synthesis technology Indra Mani 4. Advances, challenges, and opportunities in DNA sequencing technology Francisco J. Enguita and Ana Lucia Leitao 5. Recent developments in genome design and assembly tools Carlos Barreiro and Carlos Garcıa-Estrada 6. Design, building, and challenges in synthetic genomics Mansoor A. Siddiqui, Ashima Sharma and Syed Shams Yazdani 7. Synthetic minimal cells and their applications Ali Yazdizadeh Kharrazi, Amir Zare, Nils Chapin, Seyedkhashayar Ghavami and Amir Pandi 8. Small RNA-based systems for sensing and therapeutic applications Robert Penchovsky, Georgi Y. Miloshev, Nikolet Pavlova, Katya B. Popova, Aikaterini Valsamatzi-Panagiotou, Lozena A. Otcheva and Martina Traykovska 9. High-throughput navigation of the sequence space Alaksh Choudhury 10. Advances in protein engineering and its application in synthetic biology Rongming Liu, Liya Liang, Maria Priscila Lacerda, Emily F. Freed and Carrie A. Eckert 11. Computational tools for design of synthetic genetic circuits Archit Devarajan, Dhwani Gupta, Kushika Mitra, Shalini S. Deb and Shamlan M.S. Reshamwala 12. Computational tools for synthetic gene optimization Numaan Cheema, Georgios Papamichail and Dimitris Papamichail 13. De novo design and synthesis of biomolecules Subha Sankar Paul, Debarun Dhali, Yazen Yaseen, Upasana Basu, Shilpa Pal, Nihal Mohammed and Heykel Trabelsi 14. A retrobiosynthetic approach for production, conversion, sensing, dynamic regulation and degradation of molecules Pablo Carbonell 15. Advances in engineering of bacteriophages for therapeutic applications Christian Møller-Olsen, Gurneet K. Dhanoa, Tamas Feher and Antonia P. Sagona 16. Design of synthetic biology for the detection of microorganisms Dong-Dong Yang, Jake Flood, Rongming Liu and Susu He 17. Design of synthetic biological devices for detection and targeting human diseases Nedim Haciosmanoglu, Sıla Kose, Julian Ostaku, Ilkay Cisil Koksaldi, Behide Saltepe and Urartu Ozgur Safak Seker 18. Engineering cell-based therapies Juan Luis Blazquez-Roman, Mara Pisani and Velia Siciliano 19. Development of next-generation diagnostic tools using synthetic biology Elizabeth J. Vojvoda, Logan R. Burrington and Javin P. Oza 20. Advances and application of CRISPR-Cas systems Rongming Liu, Liya Liang, Margaret Habib, Emily F. Freed and Carrie A. Eckert 21. Synthetic biology for smart drug biosynthesis and delivery Srinivasan Ganesh Kumar, Ashok Ganesan and Shashi Kumar 22. Design and applications of self-assembled soft living materials using synthetic biology Gokce Ozkul, Merve Yavuz, Nedim Hacıosmanoglu, Busra Merve Kırpat and Urartu Ozgur Safak Seker 23. Synthetic gene circuits for higher-order information processing Kathakali Sarkar and Sangram Bagh 24. Cell-free synthetic biology as an emerging biotechnology Seok Hoon Hong and Ana Serratosa Fernandez-Baca 25. Recent development and applications of xeno nucleic acids Rupesh Maurya, Nisarg Gohil, Gargi Bhattacharjee, Navya L. Lam, Khalid J. Alzahrani and Vijai Singh 26. Developments in the use of microfluidics in synthetic biology Laila A. Damiati, Safa A. Damiati and Samar Damiati 27. Implications of synthetic biology research and development: a structured ethical analysis Kevin Smith

Offers basic understanding and knowledge in several aspects of synthetic biology Covers state-of-the-art tools and technologies of synthetic biology, including promoter design, DNA synthesis, DNA sequencing, genome design, directed evolution, protein engineering, computational tools, phage design, CRISPR-Cas systems, and more Discusses the applications of synthetic biology for smart drugs, vaccines, therapeutics, drug discovery, self-assembled materials, cell free systems, microfluidics, and more