1. Fundamentals of neutron crystallography in structural biology John R. Helliwell 2. Large crystal growth for neutron protein crystallography Monika Budayova-Spano, Katarina Koruza and Zoë Fisher 3. Prospects for membrane protein crystals in NMX Samuel John Hjorth-Jensen, Esko Oksanen, Poul Nissen and Thomas Lykke-Møller Sørensen 4. IMAGINE: The neutron protein crystallography beamline at the high flux isotope reactor Flora Meilleur, Andrey Kovalevsky and Dean A.A. Myles 5. The macromolecular neutron diffractometer at the spallation neutron source Leighton Coates and Brendan Sullivan 6. Current status and near future plan of neutron protein crystallography at J-PARC Ichiro Tanaka, Toshiyuki Chatake, Satoru Fujiwara, Takaaki Hosoya, Katsuhiro Kusaka, Nobuo Niimura, Taro Yamada and Naomine Yano 7. Neutron macromolecular crystallography at the European spallation source Márton Markó, Gergely Nagy, Giuseppe Aprigliano and Esko Oksanen 8. Dynamic nuclear polarization enhanced neutron crystallography: Amplifying hydrogen in biological crystals Joshua Pierce, Matthew J. Cuneo, Ann Jennings, Le Li, Flora Meilleur, Jinkui Zang and Dean A.A. Myles 9. Implementation of the riding hydrogen model in CCTBX to support the next generation of X-ray and neutron joint refinement in Phenix Dorothee Liebschner, Pavel V. Afonine, Alexandre G. Urzhumtsev and Paul D. Adams 10. Interactive model building in neutron macromolecular crystallography Derek T. Logan 11. What are the current limits on determination of protonation state using neutron macromolecular crystallography? Dorothee Liebschner, Pavel V. Afonine, Nigel W. Moriarty and Paul D. Adams 12. Proton transfer and drug binding details revealed in neutron diffraction studies of wild-type and drug resistant HIV-1 protease Andrey Kovalevsky, Oksana Gerlits, Kaira Beltran, Kevin Weiss, David A. Keen, Matthew P. Blakeley, John M. Louis and Irene T. Weber 13. Neutron crystallographic studies of carbonic anhydrase Jacob E. Combs, Jacob T. Andring and Robert McKenna 14. Protein kinase A in the neutron beam: Insights for catalysis from directly observing protons Oksana Gerlits, Kevin L. Weiss, Matthew P. Blakeley, Gianluigi Veglia, Susan S. Taylor and Andrey Kovalevsky 15. Pyridoxal 5’ phosphate dependent reactions: Analyzing the mechanism of　aspartate amino transferase Timothy C. Mueser, Victoria Drago, Andrey Kovalevsky and Steven Dajnowicz 16. The role of hydrogen atoms in redox catalysis by the flavoenzyme cholesterol oxidase Tatiana Kerber and Alice Vrielink 17. Heme peroxidase - Trapping intermediates by cryo neutron crystallography Hanna Kwon, Tobias E. Schrader, Andreas Ostermann, Matthew P. Blakeley, Emma L, Raven and Peter C. E. Moody

Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on neutron crystallography in structural biology