This volume discusses different approaches towards understanding the brain plasticity, aging, and regeneration of the nervous system in model organisms. Chapters guide the reader through an overview of model organisms,  setup of an aquatic system, Tol2-mediated transgenesis, CRISPR/Cas9-mediated gene knock-out and knock-in, Cas13d-mediated gene knockdown, tissue clearing and expansion methods for brain and spinal cord imaging,  Histological techniques, chromatin Immunoprecipitation Sequencing (ChIP-Seq) analysis, single-cell RNA sequencing analysis, BrdU/EdU incorporation assays, brain and spinal cord injury in African killifish, and emerging technologies and future perspectives in African killifish research. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. 

Authoritative and cutting-edge, Emerging Model Organisms aims to be a useful practical guide to researches to help further their study in this field. 

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Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
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Produktdetaljer

ISBN
9781071628744
Publisert
2023-01-02
Utgiver
Springer-Verlag New York Inc.; Humana
Høyde
254 mm
Bredde
178 mm
Aldersnivå
Professional/practitioner, P, 06
Språk
Product language
Engelsk
Format
Product format
Innbundet
Antall sider
15