The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge techniques that can be adapted easily to different molecules and cell types. The protocols discussed in this volume are suitable for both novice and expert scientists.

Manipulation of Cell-Cell Adhesion Using Bowtie-Shaped Microwells.- Separation of Cell-Cell Adhesion Complexes by Differential Centrifugation.- Analysis of Neutrophil Chemotaxis.- Analysis of Leukocyte Migration Through Monolayers of Cultured Endothelial Cells.- Biochemical Purification of Pseudopodia from Migratory Cells.- Dynamic Assessment of Cell-Matrix Mechanical Interactions in Three-Dimensional Culture.- Quantitative Analyses of Cell Adhesion Strength.- Using RNA Interference to Knock Down the Adhesion Protein TES.- Assaying Calpain Activity.- Analysis of Focal Adhesions and Cytoskeleton by Custom Microarray.- Proteomic Analysis of Cell Surface Membrane Proteins in Leukemic Cells.- Bioinformatic Analysis of Adhesion Proteins.- Analysis of Integrin Dynamics by Fluorescence Recovery After Photobleaching.- Double-Hydrogel Substrate as a Model System for Three-Dimensional Cell Culture.- In Vitro Actin Assembly Assays and Purification From Acanthamoeba.

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