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Dr. Anand Krishnan is a Principal Investigator in the Department of Chemical Pathology at the University of the Free State, South Africa. He leads the Precision Medicine Integrative Nano Diagnostics (P-MIND) laboratories, focusing on the biological functions of extracellular vesicles (EVs) in synthetic biology. His research includes cell surface protein modification, biomarker discovery from liquid biopsies, and the role of circulating biomarkers in disease and stem cell biology. Dr. Krishnan has authored over 150 articles and edited eight books with major publishers. He has received a National Research Foundation rating for Next Generation Researchers in South Africa and was recognized among the Top 2% of influential scientists by Stanford University. He is an active member of the International Society of Extracellular Vesicles, the African Organisation for Research and Training in Cancer, and the American Association of Cancer Research. He also serves as an editor for the eLife journal, focusing on the Africa region. Regenerate response Dr. Vadivalagan Chithravel works as a Research Fellow in the Department of Surgery at the University of Michigan, Ann Arbor, specializing in medical science, excluding epidemiology. The Exosome Mediated Signal Transduction Mechanism is his area of expertise. His current work focuses on a glycosylation biomarker associated with an exosome pathway for the physiology of cells with HCC. In addition to being a group director and scientist at the SAARP foundation in India, he has held postdoctoral research fellow positions at Taipei Medical University and China Medical University in Taiwan. He is also an active member of the International Society of Extracellular Vesicles and the American Association of Extracellular Vesicles. Dr. Prakash Gangadaran is Principal Investigator and FOUR-Brain Korea Post-Doctoral Fellow at the Department of Biomedical Science and Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, South Korea. He has expertise in utilization of extracellular vesicles in angiogenesis, wound healing, drug delivery, molecular and nuclear imaging. He has published more than 48 research articles in international peer-reviewed journals and has authored 2 book chapters as well as 24 review articles. His research interests include extracellular vesicles, angiogenesis, wound healing and drug delivery, radiotherapies, molecular imaging and nuclear imaging. Dr. Sathish Muthu is an Assistant Professor in the Department of Orthopaedics at Government Medical College, Karur, India. He is the Co-Founder and Head of Research at Orthopaedic Research Group, a non-profit research organization dedicated to translational orthopaedic research. He is also a steering committee member of the Indian Stem Cell Study Group. and associate member of Knowledge Forum Degenerative of AO Spine. Despite being a board-certified orthopaedic surgeon, Dr. Muthu holds fellowships in spine surgery, orthopaedic rheumatology, and regenerative medicine. He has published over 275 articles in international peer-reviewed journals and has contributed 5 chapters to books on cartilage injury and regeneration. He is also an Associate Editor at various academic journals like PLOS One, Journal of Clinical Medicine, Indian Journal of Orthopaedics, Journal of Orthopaedic Case Reports. His research focuses on cartilage biology, mesenchymal stem cells, exosomes, degenerative disorders, osteoarthritis, and cartilage regeneration. Dr. Ben Peacock is Head of Research at NanoFCM Co., Ltd. He has expertise in exosomal miRNA and protein profiles of EVs from cancer cell lines by RNA-seq and mass spectrometry (ion-trap) with validation by RT-qPCR and further western blotting. In international peer-reviewed journals, he has published more than nine research articles. His research interests include EV research, but he is well established in stromal communication of cancers of the head and neck. Multiple procedures were optimized to maximize EV generation from cell lines, improve concentration and purity of EV isolations, and implement best practices for sample storage.