This book provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches that are used to quantify immune mediators and analyze their function and biological activity in cancer cells and tissues.

A Useful Guide for Analysis of Immune Mediators in Cancer by Fluorochrome (Luminex) Technique.- Analysis of Circulating HMGB1 in Human Serum.- Analysis of IL-22 and Th22 Cells by Flow Cytometry in Systemic Lupus Erythematosus.- Application of Immunohistochemistry in Basic and Clinical Studies.- Interleukin-1 (IL-1) Immunohistochemistry Assay in Oral Squamous Cell Carcinoma.- Luminex xMAP Assay to Quantify Cytokines in Cancer Patient Serum.- Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.- Analysis of IFNg-Induced Migration of Ovarian Cancer Cells.- Interleukin-8-Induced Invasion Assay in Triple Negative Breast Cancer Cells.- Interleukin-8 Induces Proliferation of Ovarian Cancer Cells in 3D Spheroids.- Detection of Ferroptosis by BODIPY™ 581/591 C11.- Methods for Studying TNFα-Induced Autophagy.- Isolation of Antibody Binders to MISIIR from a Phage Display Library by Sorting.- Measuring Chimeric Antigen Receptor T Cells (CAR T Cells) Activation by Coupling Intracellular Cytokine Staining with Flow Cytometry.- Analysis of Interleukin-4-Induced Class Switch Recombination in Mouse Myeloma CH12F3-2 Cells.- Single-Stranded DNA Aptamers against TNF and Their Potential Applications.- Probing Metabolic Changes in IFNγ-Treated Ovarian Cancer Cells.- Immunoblotting Analysis of Intracellular PD-L1 Levels in Interferon-g-Treated Ovarian Cancer Cells Stably Transfected with Bcl3 shRNA.- Flow Cytometry Analysis of Surface PD-L1 Expression Induced by IFNg and Romidepsin in Ovarian Cancer Cells.- Analysis of PD-L1 Transcriptional Regulation in Ovarian Cancer Cells by Chromatin Immunoprecipitation.- Real-Time PCR Assay for the Analysis of Alternative Splicing of Immune Mediators in Cancer.- Combined Single-Cell Measurement of Cytokine mRNA and Protein in Immune Cells.- Microscopic Methods for Analysis of Macrophage-Induced Tunneling Nanotubes.- Optogenetics: Rho GTPases Activated by Light in Living Macrophages.- High-Throughput RNA interference Screen Targeting Synthetic-Lethal Gain-of-Function of Oncogenic Mutant TP53 in Triple Negative Breast Cancer.- Discovering Transcription Factor Non-Coding RNA Targets Using ChIP-Seq Analysis.- Phylogenetic Analyses of Chemokine Receptors from Sequence Retrieval to Phylogenetic Trees.- Generation of IL17RB Knockout Cell Lines Using CRISPR/Cas9-Based Genome Editing.- Engineering Mutation Clones in Mammalian Cells with CRISPR/Cas9.

This book provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches that are used to quantify immune mediators and analyze their function and biological activity in cancer cells and tissues. Beginning with a section on the detection of immune mediators in samples, the volume continues with sections covering cytokine bioassays, the expression and regulation of immune mediators in cancer cells, and methods to navigate the enormous datasets created by modern DNA and RNA sequencing and proteomic technology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and wide-ranging, Immune Mediators in Cancer: Methods and Protocols serves as a valuable resource for biochemists, molecular biologists, cancer biologists, and immunologists, as well as for physician-scientists working in the field of immunology and cancer research.