Part I Primer of antibiotic discovery process 1. Antibacterial drug discovery: A Silent Pandemic 2. Current scenario and future prospective of drug discovery and development against bacterial enzymes 3. Clinical diagnostics of bacterial infections and their resistance to Antibiotics — Current State and Novel Enabling Technologies Implementation Perspectives 4. An Odyssey into Phylogenetic Functional Conservation of Novel Antibacterial Targets in Human Pathogens 5. Validation of drug targets using molecular methodologies and enzymatic activity assays for validation of inhibitory potential 6. Computational tools to identify potential drug targets in bacteria 7. Antimicrobial drug resistance and bypassing strategies Part II Bacterial enzyme as drug targets 8. Designing Tomorrow's Antibiotics: Cutting-Edge Strategies and Technologies 9. Inhibiting the replication by targeting topoisomerases 10. Role of beta lactamases in antibiotic drug discovery 11. Selective vs broad spectrum inhibition of novel outer membrane targets in Gram negatives 12. Ribosomal binding antibacterial agents 13. RNA polymerase: A key target for Novel Antimicrobial Therapeutic Strategies 14. Colistin resistance and strategies against superbug, where we are? 15. Deoxythymidine pathway enzymes as an antibacterial target 16. Arresting the peptidoglycan synthesis to kill the bacteria 17. Clp protease complex as a therapeutic target for tuberculosis 18. PlaF: a bacterial Lands cycle phospholipase A mediating membrane phospholipid degradation and virulence adaptation 19. Bacterial TIR domain-containing proteins as drug targets 20. Drug Repurposing: Tackling the antibiotic resistance with existing therapeutics

Provides strategies and approaches to drug design aiming at overcoming antibiotic resistance. Includes most common roadblocks in identifying novel drug targets and presents the strategies to overcome. Provides potential methods to identify new drug targets by genome mining.